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work jme337 dh5α pimr83 14 y lipolytica strains w29 mat 3  (ATCC)
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com imaris imaris rrid scr 007370 matlab  (Oxford Instruments)
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Image Search Results


Strains, plasmids, and oligonucleotides used in this study

Journal:

Article Title: Characterization of an Extracellular Lipase Encoded by LIP2 in Yarrowia lipolytica

doi:

Figure Lengend Snippet: Strains, plasmids, and oligonucleotides used in this study

Article Snippet: Media were buffered with 50 mM phosphate buffer, pH 6.8. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain (host strain) or primer Plasmid, genotype, or sequence (5′→3′) c Reference, source or location (bp) E. coli strains JME225 (DH5α) pKS- LIP2 W29a This work JME257 (DH5α) JMP5 ( ura3d1 ) This work JME259 (DH5α) JMP6 ( ura3d4 /pPOX2- LIP2 ) This work JME276 (DH5α) JMP3 ( ura3d4 ) This work JME260 (DH5α) JMP8 ( ura3d1 /pPOX2- LIP2 ) This work JME298 (DH5α) JMP13 ( ura3d1 /pLIP2- LIP2 ) This work JME300 (DH5α) JMP14 ( ura3d4 /pLIP2- LIP2 ) This work JME337 (DH5α) pIMR83 14 Y. lipolytica strains W29 MAT 3 (CLIB 89, ATCC 20460) a POld MAT a ura3-302 leu2-270 xpr2-322 3 (CLIB 139) E150 MAT a ura3-302 leu2-270 xpr2-322 his1 3 (CLIB 122) Y. lipolytica recombinant strains b JMY184 = POld-6-15 JMP6/pPOX2- LIP2 A This work JMY279 = POld-6-17 JMP6/pPOX2- LIP2 A This work JMY277 = POld- lip2 KO p lip2 KO lip2 KO This work JMY278 = POld-6-15- xpr6 KO p xpr6 KO/pPOX2- LIP2 A xpr6 KO This work JMY281 = POld-14-1 JMP14/pLIP2- LIP2 A This work JMY282 = POld-14-2 JMP14/pLIP2- LIP2 A This work JMY283 = E150-6-2 JMP6/pPOX2- LIP2 A This work Primers YlipSA GCi CCi CCi AGR GAR TGi CC 4313 YlipATG CCC GGG CCG CAG TGG CCA CAA TG A AGC TT T CCA CCA TCC TTT TCA CAG CC 3733 YlipSTOP GTT G GC GGC CGC GAA TTC GGA TCC TAA ATA AAC GAT ATT CAT TTA TTA AAG TAG ATA GTT GAG G 4806 Open in a separate window a CLIB, Collection de Levures d'Intérêt Biotechnologique, Thiverval-Grignon, France. b Transformants were named according to the strain and plasmid used, and transformants were numbered as follows: for example; POld1-6-1 (strain-plasmid-clone number) c Underlined sequences correspond to introduced restriction enzyme sites as follows: for YlipATG, Hin dIII; for YlipSTOP, Not I, Eco RI, and Bam HI.

Techniques: Plasmid Preparation, Sequencing, Recombinant